Analytical repeatability, reproducibility, and linearity
Within-run precision (repeatability) and within-laboratory precision
(reproducibility) were measured using different concentrations of
sheared A. baumannii and K. pneumonia DNA samples. The
within-run coefficients of variations (CVs) of A. baumannii andK. pneumonia were 2.7%–5.3% and 2.3%–9.5%, respectively.
The within-laboratory CVs of A. baumannii and K. pneumoniawere 5.3%–7.5% and 7.4%–9.7%, respectively (Tables 5 and 6).
Linearity was determined by a 2-fold serial dilution of the DNA
template. Figure 1 shows the regression lines that represent the linear
relationships between DNA copy numbers and concentrations of A.
baumannii (R 2 = 0.9925) and K.
pneumonia (R 2 = 0.9915), respectively. Taken
together, these results indicate that ddPCR has excellent repeatability,
reproducibility, and linearity in detecting A. baumannii andK. pneumonia in blood samples.