Genomic DNA library construction and sequencing
The plantlet GM15 generated by in vitro anther culture and
regeneration system, was selected for genome sequencing. Genomic DNA was
extracted using the Qiagen DNeasy Plant Mini Kit. DNA quality was
evaluated by agarose gel electrophoresis and its quantity determined
using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham,
MA, USA). The short-insert PCR-free genomic DNA library (300-500 bp) was
constructed following the manufacturer’s protocol (Illumina Inc., San
Diego, CA, USA) for paired-end sequencing on the Illumina HiSeq X Ten
sequencer. For SMRT sequencing, the 20-kb genomic DNA library was
costructed following the manufacturer’s protocol (Pacific Biosciences,
Menlo Park, CA, USA). The detailed description in File S1.