In vitro regeneration and validation
We collected the branches with floral buds from an elite male P.
tomentosa clone (LM50), and water-cultured in a greenhouse.
Subsequently,we performed anther induced regeneration referencing
previous study (Y. Li et al., 2013). The
ploidy of regenerated anther plantlets were analyzed using a Cell Lab
Quanta SC (Beckman Coulter, CA, USA) and chromosome counting.
Furthermore, The genotype of anther plantlets were identified using 19
pairs allele-specific primers (Table S1). The detailed description was
attched to supporting information (File S1).