Experimental Approach:
The mouse neuromuscular junction of m. Levator Auris Longus was chosen
as the model of the cholinergic synapse. ACh release was assessed by
electrophysiological methods. Changes in the calcium transients were
recorded using a calcium-sensitive dye. Functional interaction between
nicotinic ACh receptors and calcium channels was investigated
pharmacologically using specific agonists and antagonists.