2.3 Genomic DNA extraction
Tissue samples were used for DNA extraction by standard procedure.
Briefly, the samples were lysed in lysis buffer, extracted with
phenol-chloroform-isoamyl alcohol (25:24:1) twice, followed by
precipitation of DNA with absolute isopropanol, washed with 70%
ethanol, dried at room temperature and resuspended in nuclease-free
water.