Figure 4. Cytokine production and transcription factor expression in Tr17 cells
a) Lymphocytes in draining lymph node were harvested from C57BL/6-Foxp3 GFP reporter mice 10 days after first CII immunization. CD4+GFP+ Treg cells and CD4+GFP- T cells were isolated and stimulated with TCR stimulation in vitro . The expression of IL-10 and IL-17 in RORγt+GFP+ Tr17 cells was analyzed using flow cytometry and compared with that in RORγt-GFP+ cells and RORγt+GFP- cells. b) At 10 days after CII immunization, Blimp-1, Foxp3, and RORγt expression in Tr17 cells, RORγt-Treg cells, and RORγt+non-Treg cells in lymph node were analyzed using flow cytometry.