Figure 1. Suppression of CIA in
Foxp3creRORγtfl/fl mice
a) Lymphocytes from the draining lymph nodes were harvested from
Foxp3wtRORγtfl/fl mice (n = 5) and
Foxp3creRORγtfl/fl mice (n = 5) at
10 days after immunization of type II collagen (CII). The expression of
RORγt in Foxp3+Treg cells was analyzed using flow
cytometry. b-c) Foxp3wtRORγtfl/flmice (n =13) and Foxp3creRORγtfl/flmice (n = 17) were immunized intradermally with CII emulsified with
complete Freund’s adjuvant (CFA) on days 0 and 21. Incidence (b) and
severity (c) of CIA were analyzed from 0 to 75 days after the first
immunization of CII. Data were obtained from three independent
experiments. d)
At
56 days post first CII immunization, joint pathology was evaluated on
decalcified hematoxylin and eosin-stained sections. Inflammation and
bone erosion scores were assessed in both groups. e) At 75 days post
first CII immunization, serum samples were collected from
Foxp3wtRORγtfl/fl mice (n = 7) and
Foxp3creRORγtfl/fl mice (n = 6) for
measurement of CII-specific total IgG levels.