Figure 4. Enrichment analysis of Gene Ontology (GO) terms and KEGG pathways
(a ) The number of significantly enriched GO terms obtained against the top 500 DEG sequences. The treatment contrasts are represented with similar colors. The GO terms are categorized into Biological process (BP), Molecular function (MF) and Cellular process (CC). (b ) Number of enriched GO terms obtained from direct count of sequences in Biological process (BP) category. (c )The KEGG metabolic pathways significantly enriched in red vs control and (d ) blue vs control. corresponding circle sizes represent the counts of sequences with p-value <0.01.
Figure 5. DEGs from light signaling and circadian rhythm. Schematic representation of red and blue light signal perception by higher plants which are likely to be involved in light regulated anthocyanin biosynthesis. The associated DEGs from red and blue light vs control contrasts were represented in two color code boxes based on log2 fold changes. Red light treatment is shown left and blue light treatment on the right side of the box.Abbreviations and gene definitions: PHYA/B, Phytochrome; CRY1/2, Cryptochrome; COP1, constitutive photomorphogenic; Elf3, early flowering 3; FT, flowering locus T; PRR5, pseudo response regulator 5; CK2α, casein kinase II subunit alpha; Cab, chlorophyll a/b binding protein; Exp, expansin; HY5, elongated hypocotyl; FKF1- flavin-binding kelch domain F box protein; SPA, suppressor of PhyA; HY5, elongated hypocotyl 5; CDF1, cycling DOF factor 1; Co, Constans.
Figure 6. DEGs from anthocyanin, carotenoid and ABA biosynthesis. Schematic representation of bilberry anthocyanin biosynthetic pathway branching from phenylpropanoid biosynthesis (top) and representation of carotenoid biosynthetic pathway leading towards abscisic acid (ABA) biosynthesis and catabolism. DEGs from flavonoid, carotenoid-ABA pathway genes and selected TFs visualized as heatmap based on log2 fold changes obtained from light treatments against the control samples. Enzyme abbreviations: PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate:CoA ligase; CCoAOMT, caffeoyl-CoA O-methyltransferase; Prx,cPrx- peroxidases, cationic peroxidases; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3-hydroxylase; F3’H, flavonoid 3’ hydroxylase; F3’5’H, flavonoid 3’5’ hydroxylase; DFR, dihydroflavonol 4-reductase; ANS, anthocyanidin synthase; UFGT, UDP-glucose flavonoid 3-O -glucosyltransferase; PSY, phytoene synthase; ZDS, zeta-carotene desaturase; BCH, beta-carotene hydroxylase; CYP 450-BCH, carotenoid β -ring hydroxylase of cytochrome P450 family; LUT1, lutein deficient 1; NCED, 9-cis -epoxycarotenoid dioxygenase; ABA 8’ hyd, Abscisic acid 8’ hydroxylase, PYR/PYL, Pyrabactin-resistance like.
Figure 7. DEGs from SNARE mediated vesicular trafficking.Schematic representation of vesicular transport of anthocyanins mediated by SNARE proteins interaction through transmembrane - Endoplasmic reticulum (ER) - golgi network. The associated transporter genes from red and blue light vs control contrasts DEGs were represented in color code boxes based on log2 fold changes. Red light treatment is shown left and blue light treatment on the right side of the box.Abbreviations and gene definitions: Stx, syntaxin-like; Bos1, Gos, Qb type golgi SNAP receptor complex; Ykt6, Sec22, vesicle transporter/VAMP like protein; SNAP, soluble NSF attachment protein; SNARE, ‘SNAP REceptor’.