Real-Time Quantitative PCR
Total RNA was extracted from peripheral blood with the Blood Total RNA Miniprep Kit (Axygen). The cDNA was transcribed by the Transcriptor cDNA Synth. Kit (Roche). Levels of targeted mRNA were determined using a Bio-rad CFX96 Touch (BIO-RAD) machine. The results of each gene were determined with the 2−ΔΔCt method, and data was expressed as fold induction. Primers were listed in Supplementary table 2.