Construction of plasmids
Full length human TYK2 cDNA template (NM003331) was purchased from
YouBio (G114136), Shanghai China and subcloned to 7.1-3×Flag-CMV
expression plasmid as wild type TYK2 (WT) using Seamless Cloning kit
from Biomed (CL116), Beijing China. Various missense and nonsense
mutation mutants of TYK2 were generated by PCR method using WT as
template. Mutations were confirmed by Sanger sequencing.