Figure 3. Analysis of cellular response to IL-6 and IL-10 in TYK2- deficient patients.
(A, B and C) Total STAT3 and pSTAT3 protein level were analyzed by western blot in P1, P2, P3 and healthy control. The patients’ PBMC were stimulated with IL-6 (100ng/μL) or IL-10 (100ng/μL) for 15 min. Representative images from 2-3 independent experiments.
(D) SOCS3 induction was analyzed by RT-qPCR after 6 h of treatment with 100 ng/ml IL-10, in PBMC from healthy controls and P3. Data are derived from 2-3 independent experiments.