Production of MSTN mutated cattle using CRISPR–Cas9
Gyeong-Min Gim1,2, Dong-Hyuk Kwon1,2, Kyeong-Hyeon Eom1,2, JoonHo Moon3, Ji-Hyun Park3, Won-Wu Lee3, Dae-Jin Jung4, Dae-Hyun Kim4, Jun-Koo Yi4, Jae-Jung Ha4, Ka-Yeong Lim5, Jin-Soo Kim5, Goo Jang1,2
1Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul National Univeristy,
2BK21 Plus program, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
3LARTBio Inc., Seoul, Republic of Korea
4Gyeongsangbukdo Livestock Research Institute, Yeongju, GyeongSang Buk-Do 36052, Republic of Korea
5Center for genome engineering, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea
Correspondence: Goo Jang, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 08826
E-mail:snujang@snu.ac.kr
Tel: 82-2-880-1280, Fax: 82-2-873-1269
Keywords: Bovine embryos, CRISPR-Cas9, In vitro fertilization,MSTN , Microinjection
Abbreviations: MSTN, myostatin; sgRNA, single guide RNA; ART, advanced reproductive technologies; SCNT,somatic cell nuclear transfer; T7E1, t7 endonuclease 1