RT-PCR
A nasopharyngeal swab sample was obtained by a trained technician and RNA was extracted with a Real Genomics viral nucleic acid extraction kit (Cat.No.YVN50/YVN100). The Novel Coronavirus (2019-nCOV) Nucleic Acid Diagnostic ‎Kit (PCR-Fluorescence Probing), Sansure Biotech (S3102E), made in Changsha, China was used for ‎qualitative detection of the ORF1ab and N genes of 2019-nCOV with a cycle ‎threshold of less than 35 (Ct<35) for positive control and Ct>40 for negative control at channel ‎FAM, ROX and CY5 (internal control) according to kit instructions. ‎