RT-PCR
A nasopharyngeal swab sample was obtained by a trained technician and
RNA was extracted with a Real Genomics viral nucleic acid extraction kit
(Cat.No.YVN50/YVN100). The Novel Coronavirus (2019-nCOV) Nucleic Acid
Diagnostic Kit (PCR-Fluorescence Probing), Sansure Biotech (S3102E),
made in Changsha, China was used for qualitative detection of the
ORF1ab and N genes of 2019-nCOV with a cycle threshold of less than 35
(Ct<35) for positive control and Ct>40 for
negative control at channel FAM, ROX and CY5 (internal control)
according to kit instructions.