3.5. LASSO capture of Human ORFs
Finally, we evaluated the ability of these manufactured LASSO probes
purified from a Cre-mediated plasmid production process to capture two
individual full-length ORFs from a cDNA derived from different human
tissues. The ~ 1 kb sequences of β -actin and
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes were successfully
captured in this manner (Fig. 5b ), as confirmed by Sanger
sequencing. The evolved method to produce LASSO probes did not impact
the ability of these probes to capture human cDNAs as was previously
shown.