3.5. LASSO capture of Human ORFs
Finally, we evaluated the ability of these manufactured LASSO probes purified from a Cre-mediated plasmid production process to capture two individual full-length ORFs from a cDNA derived from different human tissues. The ~ 1 kb sequences of β -actin and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) genes were successfully captured in this manner (Fig. 5b ), as confirmed by Sanger sequencing. The evolved method to produce LASSO probes did not impact the ability of these probes to capture human cDNAs as was previously shown.