Modeling outline
The outline of our modeling workflow in CASP14 (Figure 1) was largely
the same as in CASP1317, but with several
improvements. During the initial step, for every target we attempted to
identify multimeric templates for comparative (homology) modeling using
sequence- and structure-based search methods. If the identified
templates were not reliable, we used template-based docking. Coiled-coil
protein structures were predicted using a custom designed procedure. If
no templates could be identified, we switched to free docking. In the
case of large multisubunit targets, we combined all the methods,
employing comparative modeling approach for the parts of the complex for
which templates were available followed by docking of the resulting
subcomplexes to obtain the full assembly.