Clinical course of EAN
All mice immunized with P0 peptide 180-199 in combination with FCA
acquired EAN. In our experiment, the onset of clinical symptom of EAN in
mice occurred on day 10 p.i. After the peak phase (at day 15 p.i.), the
scores of severity of EAN decreased. On day 14 EAN mice started to
recovery. The other mice immunized with PBS and complete Freund’s
adjuvant (FCA) as the controls did not observe the symptoms of EAN
(Figure 1 ).