2.3 Whole genome sequencing of M. bovis from deer
DNA samples of M. bovis isolates from deer were sent to Oswaldo
Cruz Institute (Fiocruz), Manguinhos, Rio de Janeiro, Brazil, for WGS.
The concentration and quality of DNA were measured using Qubit
fluorometer (Invitrogen, California, USA) and Nanodrop 2000c
spectrophotometer (Thermo Fisher Scientific, Massachusetts, USA).
Paired-end genomic libraries were constructed using Nextera DNA Flex
Library Prep kit (Illumina, California, USA), according to the
manufacturer’s instructions. Genomic libraries were then sequenced in
HiSeq 2500 platform (Illumina) using HiSeq Rapid SBS Kit V2 (200
cycles). Illumina sequencing reads were made available in the Sequence
Read Archive (SRA), NCBI (Appendix Table 1).