Cell culture
The colon 26 murine adenocarcinoma cell line (C26) and Mouse colon
carcinoma (CT26) cells were cultured as a monolayer in RPMI-1640 medium
(Life Technology, NY) supplemented with 10% fetal bovine serum,
L-glutamine, and 100 units/ml penicillin and streptomycin (HyClone,
Logan, UT in a humidified atmosphere containing 5% CO2 at 37 °C. Murine
C2C12 myoblasts obtained from the American Type Culture Collection
(ATCC) were maintained in Dulbecco’s modified Eagle medium (DMEM)
supplemented with 10% fetal bovine serum and antibiotics (100 U/ml
penicillin and streptomycin; referred to as growth medium, GM). To
induce myotube differentiation, cells were grown to 100% confluence and
exposed to DMEM containing 2% horse serum (Life Technology, NY)
(referred to as differentiation medium, DM) for up to 4 days.