Cell culture
The colon 26 murine adenocarcinoma cell line (C26) and Mouse colon carcinoma (CT26) cells were cultured as a monolayer in RPMI-1640 medium (Life Technology, NY) supplemented with 10% fetal bovine serum, L-glutamine, and 100 units/ml penicillin and streptomycin (HyClone, Logan, UT in a humidified atmosphere containing 5% CO2 at 37 °C. Murine C2C12 myoblasts obtained from the American Type Culture Collection (ATCC) were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum and antibiotics (100 U/ml penicillin and streptomycin; referred to as growth medium, GM). To induce myotube differentiation, cells were grown to 100% confluence and exposed to DMEM containing 2% horse serum (Life Technology, NY) (referred to as differentiation medium, DM) for up to 4 days.