2.2. Nsp1-ribosome complex and Residue interaction networks (RINs)
PRODIGY web server [27] was used to predict the binding affinity of Nsp1-ribosome complex from it 3D structure. The residue interaction networks (RINs) approach was used to investigate key residues of Nsp1 that could play a role in interaction with the ribosome. The network was computed and visualized by RING 2.0[28,29] and Cytoscape, respectively. This network represents nodes (e.g. residues) and edges (e.g. bonds between the residue side chains), which are essential factors to network analysis.