3.4.3 Sex pheromone production
Analyses using GCMS did not detect sex pheromones from the female gametes of parthenogenetic population p28 (Figure. 8). The compounds predicted as brown algal sex pheromones were detected only from the positive control (female gametes from sexual populations p15) with high intensity at around 12.1 and 14.6 min (Figure. 8). In the similarity search for the first compound, the first candidate was 6-[(1Z )-1-butenyl]-1,4-cycloheptadiene [ectocarpene (Maier & Müller, 1986); similarity value = 97%] and the second candidate was 6-[(1E )-1-butenyl]-1,4-cycloheptadiene (thecis-trans isomer of ectocarpene; similarity value = 93%). For the second compound, the first candidate was ectocarpene (similarity value = 95%) and the second candidate was (3E ,5E ,8E )-1,3,5,8-undecatetraene (similarity value = 91%).