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Figure Legends:
Figure 1: Mitochondrial markers following
ischemia-reperfusion with age. Representative western blots of
mitochondrial markers pre- and post-operative in atrial samples of young
and aged patients (A ). Graphical representation of changes in
protein content of VDAC (B ), citrate synthase (C ), COX
I (D ), COX IV (E ), and UQCRC2 (F ).
Correlation between UQCRC2 protein expression and age (G).Ponceau stain was used for normalization of the data. #, effect of
ischemia p<0.05; *, effect of age p<0.05; Y, young;
A, aged; n=2-8. VDAC, voltage dependent anion channel; COX I, cytochrome
oxidase subunit I; COX IV, cytochrome oxidase subunit IV; UQCRC2,
ubiquinol-cytochrome c reductase core protein 2.
Figure 2: Autophagy markers following
ischemia-reperfusion with age. Representative blots of autophagy
markers pre- and post-operative in atrial tissues of young and aged
patients (A ). Graphical representation of changes in protein
content of the mature form of LC3, LC3-II (B ), ratio of LC3-II
to I (C ), p62 (D ), Optineurin (E ), and
Beclin-1 (F ). Correlation between age and Optineurin protein
content based on western blot data (G ). Ponceau stain was used
for normalization of the data. ##, effect of ischemia
p<0.01; Y, young; A, aged; n=2-11. LC3, microtubule-associated
proteins 1A/1B light chain 3B; p62, sequestosome 1.
Figure 3 : Mitophagy markers following
ischemia-reperfusion with age. Representative blots of mitophagy
markers pre- and post-operative in atrial tissues of young and aged
patients (A ). Graphical representation of observed changes in
protein content of Parkin (B ), NIX (C ). Correlation
between Parkin protein content and age (G ). Ponceau stain was
used for normalization of the data. φ, interaction effect
p<0.05; #, effect of ischemia p<0.05; ##, effect
of ischemia p<0.01; **, effect of age p<0.01; ***,
effect of age p<0.001; Y, young; A, aged; n=2-11.
Figure 4: Lysosomal markers following
ischemia-reperfusion with age. Representative blots of lysosomal
markers pre- and post-operative in atrial tissues of young and aged
patients (A ). Graphical representation of changes in protein
content of TFEB (B ), TFE3 (C ), immature Cathepsin D
(D ), and v-ATPase (E ). Graph of the relationship
between TFEB protein content (F ), TFE3 protein content
(G ) and age. Ponceau stain was used for normalization of the
data. φ, interaction effect p<0.05; φφ, interaction effect
p<0.01; #, effect of ischemia p<0.05; ##, effect
of ischemia p<0.01; *, effect of age p<0.05; **,
effect of age p<0.01; ***, effect of age p<0.001; Y,
young; A, aged; n=2-11. TFEB, transcription factor EB; TFE3,
transcription factor E3; v-ATPase, vacuolar-type H+ATPase.
Figure 5: Stress response markers following
ischemia-reperfusion with age. Representative blots of stress response
markers pre- and post-operative in atrial tissues of young and aged
patients (A ). Graphical representation of changes in protein
content of HSP70 (B ), Caspase-3 middle band (C ),
Caspase-3 top band (D ). Ponceau stain was used for
normalization of the data. Y, young; A, aged; n=5-11. HSP70, heat shock
protein 70.
Table 1 : Differential responses to IR in young and aged
patients. Summary of how proteins respond following IR in human atrial
tissues and how age influences the overall stress response. This wide
sampling of proteins illustrates a blunted response in the aged
patients, which may influence the degree of recovery to IR.