5
CONCLUSION
The present study presented the first data investigating T.
gondii genotyping from Gallus gallus domesticus, and revealed the
existence of a large amount of atypical genotypes, in special in South
America. Some studies show new genotypes never described before, which
reinforces the idea that some years from now, even more new genotypes
will be isolated, due to progressive genetic recombination.
The large amount of not defined genotypes due to inconclusive markers
suggests the need of Nested-PCR technique in genotyping studies. The
knowledge of the genotyping of the strains prevailing in free-range
chickens denotes the virulence patterns found in each continent. The
lack of data in Continents such as Europe, Asia and Oceania makes it
necessary to perform new isolating and genotyping studies in these
places.