3.4 Multiloci analysis of Toxoplasma gondii genotypes defined by network phylogeny
A total of 68 RFLP genotypes (ToxoDB) were recognized among the 561 isolates’ DNAs analyzed from the 30 studies. Phylogenetic network was inferred by the software SplitsTree4 (Huson e Bryant, 2006) using the coded genotyping data for 11 multiloci RFLP-PCR markers and 1 apicoplast marker (Figure 4 ). Most of the samples were clustered in three genetic groups, according to kinship with control samples (GT1-Type I, PTG-Type II and CTG-Type III). Most of the strains are related to Types I and III, and some from Type II common ancestry.