2.4.1. Soil physico-chemical properties, soil essential
nutrients and soil enzyme study
Five soil sub-samples were collected from chickpea plant rhizosphere
area of each plot of the experiment field and mixed together to form a
composite soil and it considered as one sample from each replicated plot
of field. After collecting the soil immediate put in the sterilised
plastic bag in ice box. The pot soils were collected in same process
only single pot soil collected as single replication. Soil
physicochemical property like pH (Jackson, 1958), electrical conductance
(EC) (Chopra and Kanwar, 1982), organic carbon (OC) (Walkley and Black,
1934); organic matter (OM) were measured. The soil essential nutrient
like available N (Subbiah and Asija, 1956); available P (Olsen et al.,
1954) and available K (Hanway and Heidel 1952), important soil enzyme
activity like dehydrogenase (Casida et al. 1964); urease (Kandeler and
Gerber 1988); alkaline phosphatase (Eiazi and Tabatabai 1977) and
Beta-glucosidase (Eivazi and Tabatabai, 1988), were measured from the
soil sample that were collected from the experimental field and pot
during flowering time.