Testing for allergen specific IgE
The s-IgE antibodies were tested at the age of 5 years against a mixture of airborne and food allergens (a multi-allergen test Phadiatop®, Thermo Fisher Scientific, Uppsala, Sweden) using the ImmunoCAP® enzyme immunoassay (Thermo Fisher Scientific, Uppsala, Sweden) according to the manufacturer’s protocol. The test was regarded positive if the food or inhalant allergen mixture showed a result of 0.35 kU/L or more. Individual s-IgE results were available for cow’s milk, hen’s egg, fish, wheat, house dust mite, cat, birch, and timothy grass.