2.3. Sand fly DNA extraction
Genomic DNA was extracted from the head, thorax and attached anterior abdomen of individual P. sergenti males and females (Martín-Sánchez et al., 2000). A commercially available kit was used (RealPure kit from REAL (Ref. RBMEG01), according to the manufacturer instructions. Each sandfly was individually placed in a sterile 1.5 ml Eppendorf tube and kept in liquid nitrogen for a few seconds to facilitate the mechanic rupture of the tissues using a pestle. The DNA was resuspended in 20 µl of bidistilled water and kept at -20 °C until use.