GLUT1, MPC1, GLUD1, PEDF, p-Erk1/2, Erk1/2, and HIF-1α
expressions were determined with Western blot analysis
After recovery, SKOV3 cells were adapted with cell lysis buffer (Cell
signalling Technology, London, UK) to extract proteins. The protein
samples were mixed with NuPAGE LDS sample buffer (ThermoFisher, Paisley,
UK) and boiled at 95°C for 10 minutes. The boiled samples were loaded
onto a NuPAGE Bis-Tris gel (ThermoFisher, Paisley, UK) for
electrophoresis. After electrophoresis, the protein on the gel was
transferred to a nitrocellulose membrane (ThermoFisher, Paisley, UK).
The membrane was blocked with 5% non-fat milk for 1 hour and incubated
with primary antibody at 4°C overnight and secondary antibody on the
next day (Table S1 ) for 1 hour and adapted with luminol reagent
solution A and B (Santa Cruz Biotechnology, Dallas, Texas, USA). The
protein bands were detected by GeneSnap Version 7.1 (Syngene, Cambridge,
UK) and the levels of protein were analysed by Fiji (ImageJ 2.0)
software (National Institutes of Health, Bethesda, Maryland, USA).