Cell migration was assessed with wound healing assay
SKOV3 cells were seeded in petri dishes to form a continuous monolayer. Cancer cells were incubated in pure media, 2.5% sevoflurane, or 4 μg/mL propofol for 2 hours. The monolayer of cancer cells was scratched to form a cell-free gap. The petri dishes were washed with PBS to discard suspended cancer cells. The cell-free gap was captured under a microscope as the baseline. After incubated with culture media for 24 hours, the cell-free gap was captured again under the microscope to calculate the migrate capability of cancer cells with Fiji (ImageJ 2.0) software (National Institutes of Health, Bethesda, Maryland, USA).