Materials
The SKOV3 cell line was purchased from ECACC, Wiltshire, UK. The Gibco RPMI media 1640, penicillin-streptomycin, methanol, NuPAGE LDS sample buffer, NuPAGE Bis-Tris gel and nitrocellulose membrane were obrained from ThermoFisher, Paisley, UK. The sevoflurane was purchased from ABOTT, Sittingbourne, UK. The propofol, Triton X-100, donkey serum and crystal violet were acquired from Sigma-Aldrich, Dorset, UK. The intralipid, PBS, paraformaldehyde, anti-Ki-67 antibody and luminol reagent solution were from Santa Cruz Biotechnology, Dallas, Texas, USA. The anti-rabbit and anti-mouse antibodies for immunofluorescent staining, anti-GLUT1, anti-GLUD1, anti-PEDF, anti-CXCL12 and anti-CXCR4 antibodies were obtained from Abcam, Cambridge, UK. The DAPI mounting media was acquired from Vector Laboratories, Burlingame, California, USA. The Corning Matrigel matrix was purchased from Corning, New York, USA. The anti-MPC1 and anti-HIF-1α antibodies were obtained from Novus Biologicals, Abingdon, UK. The anti-p-Erk1/2 and anti-Erk1/2 antibodies, anti-rabbit and anti-mouse antibodies for Western blot analysis, and cell lysis buffer were acquired from Cell signalling Technology, London, UK. The anti-GAPDH antibody was purchased from Merck, Hertfordshire, UK. The NMR tube was from Bruker Corporation, Rheinstetten, Germany.