GLUT1, MPC1, PEDF, HIF-1α, CXCL12, and CXCR4 expressions
were detected with immunofluorescent staining
After recovery, SKOV3 cells were washed with phosphate-buffered saline
(PBS) (Santa Cruz Biotechnology, Dallas, Texas, USA) and incubated in
4% paraformaldehyde (Santa Cruz Biotechnology, Dallas, Texas, USA) for
15 minutes. Cells were washed with PBS plus 0.02% Triton X-100
(Sigma-Aldrich, Dorset, UK) 3 times for 5 minutes each and blocked with
10% donkey serum (Sigma-Aldrich, Dorset, UK) for 30 minutes. After
blocking, cells were incubated with primary antibody at 4°C overnight
and secondary antibody for 1 hour at room temperature under dark in
sequence (Table S1 ). The 4’,6-diamidino-2-phenylindole (DAPI)
mounting media (Vector Laboratories, Burlingame, California, USA) were
mounted on cancer cells. The expressions of proteins were detected by a
fluorescence microscope.