Fig 3. The metabolomics study of sevoflurane- or propofol-treated ovarian cancer cells using 1H-NMR spectroscopy.
Ovarian cancer cells were administered with media (naïve control), intralipid (vehicle control), 2.5% sevoflurane or 4 μg/mL propofol for 2 hours plus 24 hours recovery time. After treatment, the culture media of ovarian cancer SKOV3 cells was collected for 1H-NMR spectroscopy experiment. PCA scores plot of 1H-NMR spectra of media was shown (A). R2X represented the fraction of variance in the 1H-NMR data modelled by two principal components (t[1] and t[2]). The OPLS-DA scores plots (B: NC vs . S; D: VC vs . P) and loadings plots (C: NCvs . S; E: VC vs . P) were derived from1H-NMR spectral data (n = 10). The colour bar indicated the correlation coefficient values (r2) to be high in red and low in blue. NC: naïve control group; VC: vehicle control group; S: sevoflurane group; P: propofol group; PCA: principal component analysis; OPLS-DA: orthogonal projection to latent structures-discriminant analysis; Glc: glucose; Lac: lactate; Pyr: pyruvate; Gln: glutamine; Ace: acetate; Ala: alanine; IPA: isopropanol; Val: valine; Leu: leucine; Eth: ethanol; Gro: glycerol; Asn: asparagine; FA: fatty acids; Suc: succinate; Acn: acetone; Arg: arginine; Ile: isoleucine.