GLUT1, MPC1, GLUD1, PEDF, p-Erk1/2, Erk1/2, and HIF-1α expressions were determined with Western blot analysis
After recovery, SKOV3 cells were adapted with cell lysis buffer (Cell signalling Technology, London, UK) to extract proteins. The protein samples were mixed with NuPAGE LDS sample buffer (ThermoFisher, Paisley, UK) and boiled at 95°C for 10 minutes. The boiled samples were loaded onto a NuPAGE Bis-Tris gel (ThermoFisher, Paisley, UK) for electrophoresis. After electrophoresis, the protein on the gel was transferred to a nitrocellulose membrane (ThermoFisher, Paisley, UK). The membrane was blocked with 5% non-fat milk for 1 hour and incubated with primary antibody at 4°C overnight and secondary antibody on the next day (Table S1 ) for 1 hour and adapted with luminol reagent solution A and B (Santa Cruz Biotechnology, Dallas, Texas, USA). The protein bands were detected by GeneSnap Version 7.1 (Syngene, Cambridge, UK) and the levels of protein were analysed by Fiji (ImageJ 2.0) software (National Institutes of Health, Bethesda, Maryland, USA).