Sex-specific sequence polymorphism in Macquarie perch, but not its
three relatives, revealed by amplicon sequencing
For Macquarie perch, genotypes for the ~400-bp region of
scaffold 633 captured by amplicon sequencing using F2-R3 primers were
consistent with WGS genotypes for the two females and two males from
Dartmouth (Appendix F, Table F1). Amplicon sequences for the two
individuals from coastal populations (Table F1) showed that only
variation at 93229 (the site chosen for the PCR-RFLP sexing assay) might
be consistently sex-linked across sequenced individuals. Genotypes for
the amplicon-sequenced fragment homologous to the Macquarie perch
sex-linked region did not reveal clear sex-linked variation in golden
perch (Table F2), Murray cod or trout cod (Table F3).