Sex-specific sequence polymorphism in Macquarie perch, but not its three relatives, revealed by amplicon sequencing
For Macquarie perch, genotypes for the ~400-bp region of scaffold 633 captured by amplicon sequencing using F2-R3 primers were consistent with WGS genotypes for the two females and two males from Dartmouth (Appendix F, Table F1). Amplicon sequences for the two individuals from coastal populations (Table F1) showed that only variation at 93229 (the site chosen for the PCR-RFLP sexing assay) might be consistently sex-linked across sequenced individuals. Genotypes for the amplicon-sequenced fragment homologous to the Macquarie perch sex-linked region did not reveal clear sex-linked variation in golden perch (Table F2), Murray cod or trout cod (Table F3).