2.4. Video and image analysis
Videos of the larvaeās locomotor response were analyzed using Kinovea,
an open-source software (www.kinovea.org,
France) to determine the RD and TBF, as reported in Eq. 1 and 2. The
automatic point tracking feature within Kinovea facilitated tail
tracking, producing an Excel file (Microsoft Corp., WA, USA) of the tail
tip coordinates. Every video frame was double checked to ensure that the
software accurately tracked the tail position. Manual adjustment was
applied as necessary if low-resolution images due to fast tail motion
hindered the automatic tail tip detection process. Any small tail flicks
were not included to be consistent with the methods reported in the
literature[16,34].
\(Response\ Duration\ (RD)=Movement\ End\ Time-Movement\ Start\ Time\ \)