Determination of the Optimal Protein Amount of p30 and p72
The p30 and p72 proteins were diluted to different concentrations and verified using a 10% NaCl destructive test. As shown in Figures 6A and 6B , the optimal labeling concentrations for p30 and p72 were 60 and 9.6 μg/mL, respectively.
Figure 6│ Determination of the optimal labeling of p30 protein (A) and p72 protein (B). A1–A6: p30 protein was diluted from 0.1 to 0.00625 mg/mL; B1–B8: The p72 protein was diluted from 0.25 to 0.001953125 mg/mL.
The structure of the colloidal gold test strip and the result from ICS
As shown in Figure 7A , the immunochromatographic strip was mainly constructed from a sample pad, absorbent pad, conjugate pad, nitrocellulose (NC) membrane, and a PVC sheet.
Figure 7 │ The structure of the test strip (A) and ICS results (B).