Evaluation of the ICS Performance
In the present study, to objectively evaluate the performance of the test strip, we verified its specificity, sensitivity, and stability. The test strips prepared in this study were used to test healthy pig serum; PRRSV, FMDV-A, FMDV-O, CSFV, and PCV-2 positive sera; and a positive control, P1 to test their specificity.
The positive control P1 was diluted with a sample diluent at ratios ranging from 1:2 to 1:512, and each solution was used as an independent sample for testing according to the steps outline in the section “ICS Result and Reaction Principle” below. The results were observed within 5–10 min and the sensitivity of the test strip was determined.
The test strips were stored at room temperature (18–25 ℃) and at 4 ℃ to determine their stability. Test strip stability was determined at 0, 1, 2, 3, 4, 5, 6, 7, and 8 months (Liu et al., 2017).