Recombinant Plasmids and Serum
African swine fever p30 and p72 proteins and p30 and p72 monoclonal antibodies were prepared in our laboratory. Forty attenuated ASFVΔ181/UK strain immune sera (immune serum with the highest blocking rate as determined by a Spanish ELISA kit was selected as the positive control P1), 14 ASFV infection inactivated sera, and 30 ASFV negative sera were provided by the African Swine Fever Regional Reference Laboratory (Lanzhou). CSFV, FMDV-A, FMDV-O, PRRSV, PCV-2 positive serum, and healthy swine serum were provided by the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.