2.2 Reagents and Instruments
NEBuffer 2.1, EnGen Lba Cas12a (LbCas12a) were purchased from New England Biolabs (MA, United States). RNase inhibitor and ssDNA reporter were obtained from TAKARA (Tokyo, Japan). Fluorescence intensity was measured by QuantStudio 5 (Applied Biosystems, Massachusetts, USA). Five Cas12a crRNAs targeting the LAMP amplification of p72 were synthesized from GenePharma (Shanghai, China). The fluorescence signal of degraded ssDNA-FQ report was visualized with a UV light transilluminator (Tiangen, Beijing, China).