2.3 Oligonucleotide primers for LAMP
Oligonucleotide primers specific for ASFV were designed based on its p72
gene. Multiple sequences of p72 were aligned using MegAlign of the
DNASTAR program (Version 7.1, DNASTAR, Madison, WI, USA). The most
conserved region of the gene was subjected to design LAMP primers using
the PrimerExplorer V4 software (http://primerexplorer.jp). The
LAMP primers comprised two outer primers F3, B3 and two inner primers
FIP, BIP. Five sets of primers were designed.