2.2 Reagents and Instruments
NEBuffer 2.1, EnGen Lba Cas12a (LbCas12a) were purchased from New
England Biolabs (MA, United States). RNase inhibitor and ssDNA reporter
were obtained from TAKARA (Tokyo, Japan). Fluorescence intensity was
measured by QuantStudio 5 (Applied Biosystems, Massachusetts, USA). Five
Cas12a crRNAs targeting the LAMP amplification of p72 were synthesized
from GenePharma (Shanghai, China). The fluorescence signal of degraded
ssDNA-FQ report was visualized with a UV light transilluminator
(Tiangen, Beijing, China).