Supplemental Figures
Supplemental Figure S1. Application plot of fluorescent LAMP
assay with 5 set of primers.
Supplemental Figure S2. Optimization of primer concentration
with the fluorescent LAMP assay. The concentration ratio of outer primer
and inner primer with 50 nM:200 nM, 50 nM:400 nM, 50 nM:600 nM, 100
nM:200 nM, 100 nM:400 nM, 100 nM:600 nM, 200 nM:400 nM and 200 nM:600 nM
was used to perform the fluorescent LAMP assay, respectively.
Supplemental Figure S3. Optimization of the fluorescent LAMP
reaction temperature. The LAMP assay was performed under
61oC, 62oC, 63oC,
64oC, 65oC, 66oC
and 67oC reaction temperature, respectively.
Supplemental Figure S4. Optimization of crRNA and ssDNA-FQ
reporter for CRISPR/cas12a cleavage report system. CRSIPR/Cas12a
reaction activity was compared between ssDNA-FQ reporter 1 and ssDNA-FQ
reporter 2 by using three different crRNA sets (crRNA 3, crRNA 4 and
crRNA 5) under two different concentration of dsDNA template (5ng/μl and
50ng/μl).
Supplementary Figure S5 . Optimization of ssDNA-FQ reporter
concentration. The concentration of 100 nM, 200 nM, 300 nM, 400 nM and
500 nM ssDNA-FQ was used to perform the CRISPR system assay,
respectively. Amplification plot of CRISPR detection assay (A) and
visualized by using UV light (B) with different concentration of
ssDNA-FQ reporter.