Figure 3. Extracellular application of BW-031 to DRG neurons inhibits sodium currents only with concomitant activation of TRPV1 channels.
a , Quantification of voltage-clamp recordings of sodium currents in TRPV1+ mouse DRG neurons pre-treated with vehicle (white), 100 µM BW-031 (green), 1 µM capsaicin (orange) or 1 µM capsaicin + 100 µM BW-031 (cyan). Capsaicin facilitates the inhibition of Nav currents in mouse TRPV1+ DRG neurons treated with BW-031. N=9-14 cells per condition, 1-way ANOVA, [F(3, 42)=4.26], p=0.01; Tukey’s post-hoc, *p<0.05. b , Quantification of the Nav current density data from TRPV1- DRG neurons pre-treated with vehicle (white), 100 µM BW-031 (green), 1 µM capsaicin (orange) or 1 µM capsaicin + 100 µM BW-031 (cyan). Capsaicin does not facilitate the block of Nav channels in mouse TRPV1- DRG neurons treated with BW-031. N=9-14 cells per group, 1-way ANOVA, [F(3,41)=0.40], p=0.75. Bars represent mean±SEM for each condition, while the individual data points are displayed as open circles.