Figure
7. BW-031 inhibits cough in guinea pigs following allergic airway
inflammation.
a , Experimental design. Guinea pigs were sensitized with
intraperitoneal and subcutaneous ovalbumin and challenged with inhaled
(inh.) ovalbumin two weeks later. One day after ovalbumin challenge, the
animals inhaled BW-031 (inhaled doses of 1.82, 7.07 or 17.6 mg/kg
BW-031) followed one hour later by inhalation of 400 mM citric acid
(yellow) for 7 minutes. Cough counts were measured for 17 minutes during
and following the citric acid challenge. b , Ovalbumin
sensitization and challenge causes lung inflammation, as measured by
immune cell counts in bronchoalveolar lavage (BAL). N=12-20 animals per
group (1:1 male:female), 1-way ANOVAs; total immune cell [F(1, 30)
=10.3], p=0.003; neutrophils [F(1, 30)=5.82], p=0.02; eosinophils
[F(1, 30)=11.9], p=0.002; monocytes [F(1, 30)=7.00], p=0.01;
lymphocytes [F(1, 30)=10.9], p=0.003; Tukey’s post-hoc,
*p<0.05, **p<0.01. Bars represent mean±SEM for each
condition, while the individual data points are displayed as open
circles. c , Inhaled BW-031 produces dose-dependent inhibition
of citric acid-evoked cough following allergic airway inflammation. N=12
animals per group (1:1 male:female), 1-way ANOVA, [F(3, 44)=7.113],
p=0.0005; Tukey’s post-hoc, **p<0.01, ***p<0.001.
Bars represent mean±SEM for each condition, while the individual data
points are displayed as open circles.