Figure 3. Extracellular application of BW-031 to DRG neurons
inhibits sodium currents only with concomitant activation of TRPV1
channels.
a , Quantification of voltage-clamp recordings of sodium
currents in TRPV1+ mouse DRG neurons pre-treated with vehicle (white),
100 µM BW-031 (green), 1 µM capsaicin (orange) or 1 µM capsaicin + 100
µM BW-031 (cyan). Capsaicin facilitates the inhibition of
Nav currents in mouse TRPV1+ DRG neurons treated with
BW-031. N=9-14 cells per condition, 1-way ANOVA, [F(3, 42)=4.26],
p=0.01; Tukey’s post-hoc, *p<0.05. b , Quantification
of the Nav current density data from TRPV1- DRG neurons
pre-treated with vehicle (white), 100 µM BW-031 (green), 1 µM capsaicin
(orange) or 1 µM capsaicin + 100 µM BW-031 (cyan). Capsaicin does not
facilitate the block of Nav channels in mouse TRPV1- DRG
neurons treated with BW-031. N=9-14 cells per group, 1-way ANOVA,
[F(3,41)=0.40], p=0.75. Bars represent mean±SEM for each condition,
while the individual data points are displayed as open circles.