2.9 In vivo nociceptive assay
Potential nociceptive reflexes mediated by BK-(1-9), BK-(1-7) and
BK-(1-5) were evaluated as previously described (Amaral et al. ,
2016). 8-12 weeks old C57Bl/6 mice were submitted to 10 µL intradermal
hind paw injection of BK-(1-9), BK-(1-7) or BK-(1-5) at a dose of 10
nmol. Saline was injected on the contralateral paw as control in the
same volume (10 µL). Animals were placed individually in acrylic cages
with wired floor and submitted to an adaptation period of 20 minutes.
Evaluation of mechanical nociceptive reflex was assessed with a
pressure-meter (Insight Instruments) by applying a crescent pressure in
the hind paw until the mice evoked a dorsiflexion withdrawn reflex. The
pressure by which this event occurred, expressed in grams (g) was
registered.