Figure legends
Figure 1. Abdominal ultrasonography images. The tumor was
present on segment 5 and showed a mosaic pattern (white arrow).
Figure 2. CT during angiography. The tumor showed low
absorption on CT during arterial portography (a) and high absorption on
CT during hepatic arteriography both in the early phase (b) and late
phase (c).
Figure 3. EOB-MRI images. The tumor showed a high signal on
early phase (a), low signal on T1-weighted hepatocyte phase (b), high
signal on diffusion-weighted imaging (c), and low signal on apparent
diffusion coefficient (d).
Figure 4. Histological findings in resected lymphocyte rich
hepatocellular carcinoma (loupe image, H-E stain). The enlarged image is
shown in the black frame. White line shows the border of the tumor and
liver background. H-E staining showed poorly differentiated tumor cells
with prominent nuclear irregularities and high degree of lymphocytic
infiltration in the background.
Figure 5. H-E staining and immunostaining images of tumor. H-E
(a), Glypican3 (b), Hepatocyte (c), EBV-encoded small RNA (EBER) (d).
Many infiltrating lymphocytes were found around tumor cells stained with
Glypican3 and Hepatocyte. EBER was negative.
Figure 6. Immunostaining images of infiltrating lymphocytes.
CD8 (a), CD4 (b), CD3 (c), CD20 (d). Both CD8 and CD4 were positive in
50%. CD3 was positive in almost 100% and CD20 was negative.
Figure 7. Immunostaining images. FOXP3 (a), Programmed cell
death 1 (PD-1) (b), Programmed cell death ligand 1 (PD-L1) (c). FOXP3
was weakly positive in CD4 positive cells. PD-1 was positive in almost
all lymphocytes. PD-L1 was positive in about 40% of tumor cells.
Figure 8. Low-power field showing immunostaining of PD-L1 (a).
High-power field showing Glypican 3 immunostaining (b) and PD-L1 (c).
Multiple small foci of PD-L1 positive inflammatory cell clusters were
founded around Glypican 3-positive tumor cells (circles).