Media Volume Media Volume Media Volume Media Volume
1 mL 2 mL 4 mL 8 mL
Wet Weight [normalized to control (1 mL)] 1.00 ± 0.09 * 1.42 ± 0.08 a 2.4 ± 0.3 * 2.0 ± 0.2
GAG/DNA [normalized to control (1 mL)] 1.00 ± 0.11 * 1.61 ± 0.22 a 3.30 ± 0.41 2.78 ± 0.23
Collagen/DNA [normalized to control (1 mL)] 1.00 ± 0.09 * 1.35 ± 0.40 2.26 ± 0.63 2.03 ± 0.69
Glucose Consumption [nmol/h/µg DNA] 15 ± 2 a 25 ± 3 a 63 ± 25 b 53 ± 13 b
Lactate Production [nmol/h/µg DNA] 28 ± 3 a 45 ± 7 a 105 ± 39 b 83 ± 21 b
YL/G (yield of lactate-on-glucose)† 1.88 ± 0.03 1.75 ± 0.09 1.74 ± 0.06 1.55 ± 0.06
Extracellular pH 6.62 ± 0.04 6.70 ± 0.04 6.86 ± 0.04 * 7.05 ± 0.05 *
O2 Consumption (qO2) [nmol/h/µg DNA] 0.23 ± 0.04 a 0.43 ± 0.05 a 0.84 ± 0.08 b 1.2 ± 0.1 b
CO2 Production (qCO2) [nmol/h/µg DNA] 4.0 ± 0.8 6.7 ± 0.5 11.5 ± 0.9 27 ± 3 *