2.3. Statistical analysis
Statistical analysis was performed using GraphPad Prism ver 8.4.1(GraphPad Software, San Diego, CA). Kruskal-Wallis test and Dunn’s multiple comparison test were used to compare the antigen-specific Ig levels of each group. The correlation between antigen-specific IgE, IgD, and IgG4 levels was analyzed by Spearman’s rank correlation test. A p-value <0.05 was considered statistically significant.