Figure 2. Comparison of culture vessel for T cell products .
Co-cultures of apheresis MNC and autologous LCLs were set up (n=6
donors) to compare culture in Corning flasks versus G-Rex100 flasks
through an optimized three stimulation round protocol. Harvested samples
were compared for phenotypic and functional profiles as before.
(A ) Cultures from G-Rex flasks had a significantly higher
percentage of CD8+ cells than standard flask grown lines
(p<0.05). (B ) CD8+ cell phenotype was consistent
between both culture methods for all markers analysed. Intracellular
analysis of the gated CD8+/CD45RO+ cells showed no difference between
standard flask and G-Rex cultured T cells for (C ) expression of
cytokines by cMFI or (D ) relative frequencies of differentiated
subpopulations by cytokine expression. Data are represented as means ±
SEM.