Spatial and temporal TLR3 variation across islands
The earliest available samples from the source population, Cousin (120
birds caught in 1993 and 1994), were used to provide a proxy estimate of
the initial TLR3 diversity on Aride and Cousine (which were
established in 1988 and 1990, i.e.. before sampling took place). Samples
from 56 of the 58 birds translocated to Denis, and all 59 birds
translocated to Frégate were used to determine initial TLR3diversity on these islands. The most recent population samples were of
58 individuals caught in 2018 on Frégate, 158 individuals caught in 2015
on Denis, 54 individuals caught in 2012 and 2016 on Aride, 72
individuals caught in 2019 on Cousine, and 196 individuals caught in
2018 on Cousin.
Genepop 4.2 (Rousset, 2008) was used to test if the different island
populations conformed to HWE (as above). We tested for temporal and
spatial divergence in TLR3 frequencies among populations using
genic differentiation tests (Raymond & Rousset, 1995) in Genepop 4.2
(Rousset, 2008). Fisher’s exact test and the Markov chain algorithm
parameters were as above. First, we tested for differentiation between
the initial (translocated or 1993–94 samples) and most recent samples
from each population. Second, we tested for differentiation among
populations using the most recent samples.