UGT1A1 genotyping analysis
UGT1A1 genotyping was performed using the Invader assay, a DNA
analysis method that consists of a two-step isothermal reaction using a
structure-specific flap endonuclease, as previously
reported12. Based on the results of testing forUGT1A1*6 and *28 genetic polymorphisms, patients were
divided into three groups: wild-type group (−/− ); heterozygous
group (*28/− and *6/− ); and homozygous group
(*28/*28 , *6/*6 , and *28/*6 ). Patients with double
heterozygosity (*28/*6 ) were classified into the homozygous
group13.