2.2.4 Statistical analysis and figure drawing
Statistical analyzes were performed using Microsoft Excel 2007, SPSS
Statistics 20.0 (IBM, Armonk, New York, USA) and R package vegan
(version 2.5-5). Adobe Illustrator CC 2017 and R package ggplot2
(version 3.2.0) were used to draw figures (Oksanen et al., 2019;
Kraemer, Ramachandran, Colatriano, Lovejoy & Walsh, 2020).
The average value of all parameters was taken from the five replicates
and the standard error was calculated. The data were analyzed by the
multiple t -test (p≤0.05). Correlation analysis used the Mantel
test and Spearman’s rank correlation coefficients (Guillot & Rousset,
2013; Sedgwick, 2014). LDA Effect Size analysis (LEfSe) used the ANOVA
and then the Wilcoxon rank sum test to analyze the differences between
groups (threshold set to 0.05) (Kuffner et al. 2012; Yue et al., 2020).