2.7. Amyloid β plaques histology
Aβ plaques were stained with Thioflavin-S. Brain coronal sections of 30 μm were obtained (Leica Microsystems CM 3050S cryostat, Wetzlar, Germany) and kept in a cryoprotectant solution at −20°C until use. Free-floating slices were placed in a 24-well plate and washed with 0.01M PBS for 5 min at room temperature to be rehydrated. Next, the brain sections were washed with 70% ethanol for 1 min followed by a wash with 80% ethanol for 1 min. The slices were then incubated with 0.3% Thioflavin-S (Sigma-Aldrich) solution for 15 min at room temperature in the dark. Afterwards, the samples were washed using 80%, 70% and 50% EtOH for 1 min each. Three 2-min washes with 0.1M PBS and the slices were mounted using Fluoromount-G (EMS, USA).