2.9 Statistical Analysis
The data and statistical analysis comply with the recommendations of the
British Journal of Pharmacology on experimental design and analysis in
pharmacology (Curtis et al., 2018). Group size may vary according to
power analysis and expertise of the authors regarding the behavioural
tests (Griñan-Ferré et al., 2016; Griñán-Ferré et al., 2018) and
statistical analysis was undertaken only for studies where each group
size was at least n=5. Blinded analysis was performed for behavioural
test.All data are expressed as the mean ± standard error of the mean
(SEM). Statistical analysis was conducted using GraphPad Prism version 8
statistical software. All data were tested for normal distribution and
equal variance. In the cognitive and behavioural studies, means were
compared with two-way ANOVA. In molecular studies, means were compared
with two-tailed Student’s t-test (WT Control vs 5XFAD Control) or
one-way ANOVA followed by Tukey’s post hoc tests (5XFAD Control vs 5XFAD
treated groups). Statistical significance was considered when P values
were <0.05. Statistical outliers were determined with Grubbs’
test and when necessary were removed.