Statistical Analyses
First, characteristics of the study population were determined,
stratified for FLG mutations . P-values for determining
differences between the categorical variables between the two groups
were calculated with chi-squared tests. Next, median cell numbers with
interquartile range (IQR) were determined. Differences in cell numbers
between children with and without FLG mutations were determined
with the non-parametric Mann-Whitney U tests. Sensitivity analyses on
the associations of FLG genotype with immune cell numbers were
performed within children who were diagnosed with AD. No adjustment for
multiple testing was performed because of strong correlation between the
immune cells studied. Statistical analyses were performed with SPSS
version 21.0 (IBM Corp.) and R version 3.3.3 (R Foundation for
Statistical Computing).