FLG genotype
DNA samples obtained from umbilical cord blood were genotyped by modified Taqman allelic discrimination assays with the use of primers as described previously.19 The following 4 FLGmutations were identified: R501X (rs61816761), S1085CfsX36 (rs41370446), R2447X (rs138726443), and S3247X (rs150597413).19,20Children were classified as having a FLG mutation if they were homozygous, compound heterozygous or heterozygous for any of the four mutations. Children without any of the four mutations were classified as wild type.