Statistical Analyses
First, characteristics of the study population were determined, stratified for FLG mutations . P-values for determining differences between the categorical variables between the two groups were calculated with chi-squared tests. Next, median cell numbers with interquartile range (IQR) were determined. Differences in cell numbers between children with and without FLG mutations were determined with the non-parametric Mann-Whitney U tests. Sensitivity analyses on the associations of FLG genotype with immune cell numbers were performed within children who were diagnosed with AD. No adjustment for multiple testing was performed because of strong correlation between the immune cells studied. Statistical analyses were performed with SPSS version 21.0 (IBM Corp.) and R version 3.3.3 (R Foundation for Statistical Computing).