Statistical analysis
For normally distributed continuous parametric variables Student’s t-test was used and expressed as mean ± standard deviation (SD). Kruskal-Wallis test was used when normal distribution was not present. Fisher exact test or the χ2 test was used for categorical variables and expressed as number and percentage. Paired Student’s t -test was used to compare means from baseline to follow-up. A p-value of <0.05 was considered statistically significant. Continuous variables of baseline demographics are expressed as mean ± SD. Results are expressed as mean ± SD. Categorical variables were expressed as number and proportion. Analysis was performed using SPSS version 24 (IBM SPSS statistics, IBM corporation, Armonk, New York).