FIGURE LEGENDS
Fig. 1: Target species study experimental design . US=
ultrasound exam; EB= estradiol benzoate; EC= estradiol cypionate; P4=
progesterone; IVD= intravaginal device.
Fig. 2: Culture of P5C3 clone in a 1 L bioreactor. Evolution of
cell density, cell viability, lactate, glucose and reCG concentration
along time. Temperature and perfusion rate per day are shown. Asterisk
character indicates cell bleeding.
Fig. 3: SDS-PAGE of fractions corresponding to different stages
of reCG purification. Samples were subjected to electrophoresis under
non-reducing conditions and stained with Coomassie blue. Lane MW, Blue
Plus® Protein Marker (Transgene Biotech); lane 1, clarified supernatant;
lane 2, flowthrough; lane 3, wash; lane 4, eluate.
Fig. 4: Western blot analysis of reCG under different
conditions. A: Unheated samples were electrophoresed under non-reducing
conditions. B: Heated samples were electrophoresed under
non‑reducing conditions. C: Heated samples were electrophoresed
under reducing conditions. After SDS-PAGE proteins were probed with
antiserum against reCG. For each panel, lane 1 corresponds to PMSG, lane
2 to reCG, lane 3 to supernatant of sCHOwt, and lane MW to
ECLTM RainbowTM Marker
(AmershamTM).
Fig. 5: IEF profile of PMSG (lane 1) and reCG (lane 2)
glycoforms. After separation, proteins were transferred onto PVDF
membrane and probed with rabbit polyclonal sera anti-reCG. The line
indicates the range of pH employed.