Figure caption
Fig. 1. Feedback feeding platform in SLs fermentation process. The solid lines represent material pipelines, dotted lines represent signal pipelines.
Fig. 2. Process parameters of SLs fermentation with different oil feeding modes. A: SLs titer; B: phased SLs productivity; C: difference in total SLs production; D: process CER and OUR profilings
Fig. 3. Metabolic characteristics of SLs at different production stages. A: The effect of the product on the synthesis of SLs; B: Changes in RQ during SLs synthesis; C: Relation between SLs concentration and viscosity.
Fig. 4. Analysis of SLs synthesis stage.
Fig. 5. Intelligent feedback feeding model. OUR for the oxygen uptake rate (mmol/L/h), CER for the CO2 release rate (mmol/L/h), RQ for the respiratory quotient, RPM for the fermentation speed (rpm), DO for the dissolved oxygen (%), Temp for the fermentation temperature (℃), pH for the fermentation pH. SLs represents the productivity of SLs (g/L/h), Oil represents the consumption rate of rapeseed Oil (g/L/h), Glc represents the consumption rate of glucose (g/L/h), V represents the working volume (L), c1 and c3 represent the concentrations of rapeseed Oil and glucose in the actual fermentation broth (g/L), c2 and c4 represent the controlling concentrations of rapeseed oil and glucose in fermentation broth (g/L).
Fig. 6. Feedback feeding control. A: Glucose concentration; B: Rapeseed oil concentration.
Fig. 7. Semi-continuous fermentation of SLs production with the assistance of intelligent feedback feed model. A: Rapeseed oil and glucose concentration as well as DCW; B: SLs titer; C: SLs productivity and yield.