4. Conclusion
In this study,
the
mechanism of SLs sedimentation in multiphase fermentation process was
elucidated for the first time. And then
UEST was introduced to strengthen
the SLs sedimentation rate.
The
real-time rational in-situ separation of SLs and semi-continuous
fermentation with UEST was accomplished to achieve productivity and
yield of 2.15 g/L and 0.58 g/g respectively as well as reduce the losses
of cells, glucose, and oil by 68.2%, 16.2%, and 65.5%, respectively.
The rational in-situ separation and UEST for complex multiphase
systems could be extended to other biosurfactant fermentation systems to
improve production efficiency.