Virus isolation
The supernatants of PiAdV-A and PiCV positive samples from a pigeon
flock were inoculated into SPF embryonated chicken eggs. All samples
were passaged 5 times in SPF chicken eggs. It was not possible to
propogate PiAdV-A virus from the PiAdV-A PCR positive sample in eggs,
but PiCV virus was propagated from the PiCV PCR positive sample in the
SPF embryonated chicken eggs and caused extensive damage to chicken
embryos regardless of the inoculation route (chorioallantoic cavity and
yolk sac) (Figure.3). PiAdV-A and PiCV positive samples did not lead to
any propogation of virus in primary CEFCs, but both samples caused
extensive rounding, clumping and detatchment of cells in the CEKC
cultures 48-72 hours post-inoculation (Figure. 4).
FIGURE 3. a-b-c) Extensive malformation and hemorrhaging was
caused in chicken embryos by PiCV (TR/SKPC20) and compared with normal
chicken embryo (d). Livers with multiple petechial haemorrhages (black
arrows)