PCR
The PiAdV-A fiber-2 and PiCV capsid genes were partially amplified by
PCR using the F1-s/F2-as and PiCV2-s/PiCV2-as primers, respectively. The
expected 967-bp and 325-bp specific DNA fragments were amplified
(Figure. 3). The specific PCR products were identified as PiAdV-A and PiCV
by sequencing and BLAST analysis.
FIGURE 5. a) Lane M, 100 bp ladder marker; lane 1, TR/SKPA20
(liver); lane2, TR/SKPA20 (kidney) ;lane 3, TR/SKPA20 (spleen); lane 4,
TR/SKPA20 (gut and pancreas); lane 5, negative control (Raue et al.,
2002) (967 bp.). Lane 6, TR/SKPC20 (liver); lane7, TR/SKPC20 (kidney) ;
lane 8, TR/SKPC20 (spleen); lane 9, TR/SKPC20 (gut and pancreas); lane
10, negative control (Freick et al., 2008) (325 bp.).