DNA sequencing and phylogenetic analysis
The amplified fiber-2 gene and capsid gene fragments were sequenced
directly. The PiAdV-A isolate was named ”TR/SKPA20” and the PiCV
isolate was named ”TR/SKPC20”. A BLAST search
(https://blast.ncbi.nlm.nih.gov) was used to compare these sequences
with the complete genomes of aviadenovirus and circovirus species. The
newly detected field isolate TR/SKPA20 was found to be related with only
one PiAdV-A ‘’IDA4” strain, with 99.03% similarity at the amino acid
level. The TR/SKPC20 field isolate was found to be closely related with
strains previously isolated in Poland (MK994767, KC691682), Hungary
(JF330097) and Taiwan (GO844278) (Figures. 6 and 7). The nucleotide
sequences of the field isolates presented here (TR/SKPA20 and TR/SKPC20)
have been submitted to the NCBI-GenBank database under the following
accession numbers: MN985817, MT130538.
FIGURE 6. Phylogenetic tree based on the amino acid sequence of
the PiCV capsid gene. The tree shows circovirus species which affect
fowl. The tree shows the names and GenBank accession numbers of each
isolate. The PiCV isolate from this study is indicated by a red dot.