DNA sequencing and phylogenetic analysis
The amplified fiber-2 gene and capsid gene fragments were sequenced directly. The PiAdV-A isolate was named ”TR/SKPA20” and the PiCV isolate was named ”TR/SKPC20”. A BLAST search (https://blast.ncbi.nlm.nih.gov) was used to compare these sequences with the complete genomes of aviadenovirus and circovirus species. The newly detected field isolate TR/SKPA20 was found to be related with only one PiAdV-A ‘’IDA4” strain, with 99.03% similarity at the amino acid level. The TR/SKPC20 field isolate was found to be closely related with strains previously isolated in Poland (MK994767, KC691682), Hungary (JF330097) and Taiwan (GO844278) (Figures. 6 and 7). The nucleotide sequences of the field isolates presented here (TR/SKPA20 and TR/SKPC20) have been submitted to the NCBI-GenBank database under the following accession numbers: MN985817, MT130538.
FIGURE 6. Phylogenetic tree based on the amino acid sequence of the PiCV capsid gene. The tree shows circovirus species which affect fowl. The tree shows the names and GenBank accession numbers of each isolate. The PiCV isolate from this study is indicated by a red dot.