Toxoflavin and UV radiation tolerance
Overnight cultures of the PA13 derivatives were sub-cultured and grown
for an additional 12 h. A 100-μL aliquot was removed and serially
diluted 10-fold, and 10-μL of each culture was spotted on LB agar plates
supplemented with 20 μg mL−1 toxoflavin. The spotted
plates were incubated at 28°C for 36 h.
For the UV radiation tolerance assays, PA13 derivatives were spotted on
LB plates using the above procedure and treated as previously described
(Mohammadi et al ., 2012).