Appendix Figure. The virus was cultured in eight different cell types (BHK-21, NA, PK-15 ST, Vero, Marc-145, MDBK, MDCK). Obvious cytopathic effects, including cellular rounding, refraction, cell death, shedding, and floating occured in the BHK-21, PK-15, ST, and Vero cells after 36 h. However, only individual cells died and detached in the other four cell types until 96 h in NA, Marc-145, MDBK, and MDCK cells.