Figure legends
Figure 1 . Enrichment of food antigen-specific T-cells.(A-C ) Peripheral blood mononuclear cells (PBMC) were stimulated
with various food antigens, magnetically enriched for CD154 and analyzed
by flow cytometry. (A ) Methodology and (B ) exemplary
density plots of CD154+ T-cell enrichment after
stimulation with gliadin or control antigen are shown. (C )
Frequencies of CD4+ T-cells in PBMC were determined
from healthy controls (non-relatives, NR), patients with active Crohn´s
disease (CD) or ulcerative colitis (UC), or each of these entities in
remission (-R), celiac disease patients (CeD) ± gluten-free diet (GFD,
aCeD) or refractory (Refr) patients, first degree relatives of CeD
patients (FDR) and rheumatoid arthritis patients (RA). Data are shown as
median with 95% CI.
Figure 2 . Phenotyping gliadin-specific T-cells.(A-F ) Peripheral blood mononuclear cells (PBMC) were stimulated
with various food antigens, magnetically enriched for CD154 and analyzed
by flow cytometry. (A ) Frequencies of CD154+cells among CD4+ T-cells in PBMC from healthy controls
(non-relatives, NR), patients with active Crohn´s disease (CD) or
ulcerative colitis (UC), or each of these entities in remission (-R),
celiac disease patients (CeD) ± gluten-free diet (GFD, aCeD) or
refractory (Refr) patients, first degree relatives of CeD patients (FDR)
and rheumatoid arthritis patients (RA) are shown. (B ) Ratio of
gliadin-specific effector memory CD4+CD154+ T-cells (CD45RA-CCR7-) and naïve T-cells
(CD45RA+CCR7+) from controls and
disease groups are shown. (C ) Exemplary dot plots of α4β1
within CD4+ and
CD4+CD154+ T-cells are shown.
(D ) Frequencies of CD4+ T cells and
CD4+ CD154+ T cells, positive for
integrins α4β1 and α4β7 are shown. (E ) Frequencies of
gliadin-specific IFNγ+, IL-17A+ and
TNFα+ cells within CD154+ T-cells in
between the patient groups are shown. (F ) Exemplary dot plots
of IFNγ+, IL-17A+ and
TNFα+ CD4+CD154+gliadin-specific T-cells are shown. Data are shown as median.
Significance was determined using Mann-Whitney-U-Test.
*P>0.05, **P>0.01, ***P>0.001.
Statistically significant differences were calculated in comparison to
healthy non-relatives, if not indicated otherwise.
Figure 3 . Frequencies of food antigen-specific T-cells.(A-B ) Peripheral blood mononuclear cells (PBMC) were stimulated
with various food antigens, magnetically enriched for CD154 and analyzed
by flow cytometry. (A ) Exemplary dot plots of
CD154+ T-cell enrichment of indicated nutritional
antigens with absolute numbers and frequencies are shown. (B )
Frequencies of CD154+ cells among
CD4+ T-cells after stimulation with soybean, peanut
and OVA-peptide from healthy controls (non-relatives, NR), patients with
active Crohn´s disease (CD) or ulcerative colitis (UC), or each of these
entities in remission (-R), celiac disease patients (CeD) ± gluten-free
diet (GFD, aCeD) or refractory (Refr) patients are shown. Data are shown
as median. Significance was determined using Mann-Whitney-U-Test.
*P>0.05, **P>0.01, ***P>0.001.
Statistically significant differences were calculated in comparison to
healthy non-relatives, if not indicated otherwise.
Figure 1