Figure legends
Figure 1 . Enrichment of food antigen-specific T-cells.(A-C ) Peripheral blood mononuclear cells (PBMC) were stimulated with various food antigens, magnetically enriched for CD154 and analyzed by flow cytometry. (A ) Methodology and (B ) exemplary density plots of CD154+ T-cell enrichment after stimulation with gliadin or control antigen are shown. (C ) Frequencies of CD4+ T-cells in PBMC were determined from healthy controls (non-relatives, NR), patients with active Crohn´s disease (CD) or ulcerative colitis (UC), or each of these entities in remission (-R), celiac disease patients (CeD) ± gluten-free diet (GFD, aCeD) or refractory (Refr) patients, first degree relatives of CeD patients (FDR) and rheumatoid arthritis patients (RA). Data are shown as median with 95% CI.
Figure 2 . Phenotyping gliadin-specific T-cells.(A-F ) Peripheral blood mononuclear cells (PBMC) were stimulated with various food antigens, magnetically enriched for CD154 and analyzed by flow cytometry. (A ) Frequencies of CD154+cells among CD4+ T-cells in PBMC from healthy controls (non-relatives, NR), patients with active Crohn´s disease (CD) or ulcerative colitis (UC), or each of these entities in remission (-R), celiac disease patients (CeD) ± gluten-free diet (GFD, aCeD) or refractory (Refr) patients, first degree relatives of CeD patients (FDR) and rheumatoid arthritis patients (RA) are shown. (B ) Ratio of gliadin-specific effector memory CD4+CD154+ T-cells (CD45RA-CCR7-) and naïve T-cells (CD45RA+CCR7+) from controls and disease groups are shown. (C ) Exemplary dot plots of α4β1 within CD4+ and CD4+CD154+ T-cells are shown. (D ) Frequencies of CD4+ T cells and CD4+ CD154+ T cells, positive for integrins α4β1 and α4β7 are shown. (E ) Frequencies of gliadin-specific IFNγ+, IL-17A+ and TNFα+ cells within CD154+ T-cells in between the patient groups are shown. (F ) Exemplary dot plots of IFNγ+, IL-17A+ and TNFα+ CD4+CD154+gliadin-specific T-cells are shown. Data are shown as median. Significance was determined using Mann-Whitney-U-Test. *P>0.05, **P>0.01, ***P>0.001. Statistically significant differences were calculated in comparison to healthy non-relatives, if not indicated otherwise.
Figure 3 . Frequencies of food antigen-specific T-cells.(A-B ) Peripheral blood mononuclear cells (PBMC) were stimulated with various food antigens, magnetically enriched for CD154 and analyzed by flow cytometry. (A ) Exemplary dot plots of CD154+ T-cell enrichment of indicated nutritional antigens with absolute numbers and frequencies are shown. (B ) Frequencies of CD154+ cells among CD4+ T-cells after stimulation with soybean, peanut and OVA-peptide from healthy controls (non-relatives, NR), patients with active Crohn´s disease (CD) or ulcerative colitis (UC), or each of these entities in remission (-R), celiac disease patients (CeD) ± gluten-free diet (GFD, aCeD) or refractory (Refr) patients are shown. Data are shown as median. Significance was determined using Mann-Whitney-U-Test. *P>0.05, **P>0.01, ***P>0.001. Statistically significant differences were calculated in comparison to healthy non-relatives, if not indicated otherwise.
Figure 1