Gene set enrichment analysis
FUMA platform (http://fuma.ctglab.nl/) was used to study the enrichment
analysis of the obtained genes. FUMA is a web-based comprehensive
platform that can utilize a variety of biological resource information
to annotate the function, gene priority and interactive visualization of
GWAS results 22. Genes with genome-wide significant
interaction effects or suggestively significant interaction effects on
left HGS or on right HGS identified in GWEIS were used as input.
GENE2FUNC function was used to obtain related gene ontology (GO).
Enrichment of prioritized genes is tested using the hypergeometric test
against gene sets obtained from MsigDB 23. A total of
5817 GO terms were included in the analyses, in which 4436 for GO
biological process, 580 for GO cellular component and 901 for GO
molecular function. Benjamini-hochberg method was performed to correct
multiple tests.