ELISA inhibition
ELISA inhibition was performed using serum before OIT from seven of the
nine patients who underwent the second OFC. Cases 3 and 9 were excluded
because of an insufficient volume of serum. The binding of IgE to wheat
extract was inhibited by more than 80% after preincubation of the serum
with a high concentration of wheat extract, whereas preincubation of
serum with a high concentration of barley extract inhibited binding of
IgE to wheat extract by less than 10% (Fig 2). On the other hand, wheat
and barley extracts equally inhibited binding to barley sIgE at high
concentrations. Furthermore, wheat exhibited more inhibition than barley
at low concentrations (0.01 mg/ml) of inhibiting extract
(p =0.03).