Funding information
National Natural Science Foundation of China, Grant/Award Numbers:
21877089, 21602177, and 21572182
Word count: 3,787 words, excluding abstract, methods,
references, and figure legends.
Acknowledgements: This research was supported by the National
Natural Science Foundation of China (21877089, 21602177, and 21572182).
We thank Dr Zhengfang Yi at East China Normal University for the
instructions.
Conflict of interest statement: The authors declare no
conflicts of interest.
Data availability statement: The data that support the findings
of this study are available from the corresponding author upon
reasonable request. Some data may not be made available because of
privacy or ethical restrictions.
Declaration of transparency and scientific rigour: This
Declaration acknowledges that this paper adheres to the principles for
transparent reporting and scientific rigour of preclinical research as
stated in the BJP guidelines for Design and Analysis,
Immunoblotting and Immunochemistry, and Animal Experimentation, and as
recommended by funding agencies, publishers and other organizations
engaged with supporting research.
Abbreviations: MTS,
3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium,
Inner Salt; PCR, Polymerase Chain Reaction; DMSO, Dimethyl Sulfoxide;
DMEM, Dulbecco’s Modified Eagle’s Medium; RPMI, Roswell Park Memorial
Institute; IMDM, Iscove’s Modified Dulbecco’s Medium; HPLC, High
Performance Liquid Chromatography; FBS, Fetal Bovine Serum; PBS,
Phosphate Buffer Saline; FITC, Fluorescein Isothiocyanate; PI, Propidium
Iodide; EDTA, Ethylenediaminetetraacetic Acid; AA, Amino Acids; NTA,
Nitrilotriacetic Acid; RIPA, Radio Immunoprecipitation Assay; IL-6,
Interleukin 6; RNA-seq, RNA sequencing; FC, Fold Change; KEGG, Kyoto
Encyclopedia of Genes and Genomes; JAK, Janus kinase