RNA extraction and gene clone
The roots and nodes of S. alterniflora (0.2 g fresh weight) were
ground into powder in liquid nitrogen. The total RNA was extracted by
using an RNA purification reagent kits and reverse transcribed into cDNA
by using M-MLV reverse transcriptase (TaKaRa, Dalian, China). The
homologous genes of different plant species obtained from NCBI database
were aligned and used to design the degenerate primers (Supplementary
Table S2). The amplified products were sequencing and the sequences were
search in NCBI database (Supplementary Table S3). After that, the
identified sequences were used to design the gene-specific primers for
quantitative real-time PCR (qRT-PCR) (Supplementary Table S4).