<div>Afterwards, the content of strigolactones and its derivatives in<i>S. alterniflora</i> roots were measured using the method of
HPLC-MS/MS. It is surprising that the 5-DS was only detected in 0‰
salinity treatment and strigol was detected in 15‰ salinity treatment,
while none of them could be detected in 30‰ salinity treatment. Even so,
the content of 5-DS in 0‰ salinity treatment (0.1416 ± 0.0225 ng/g) was
higher than the content of strigol (0.0632 ±0.0044 ng/g) in 15‰ salinity
treatment (Table 1).</div><div>Furthermore, four genes which participate in strigolactone biosynthesis
(<i>D17</i> , <i>D10</i> ) and signal transduction (<i>D14</i> ,<i>D53</i> ), were cloned from <i>S. alterniflora</i> . The fragment of
these four genes were amplified by using the degenerate primers
(Supplementary Table S2) and the sequences were acquired (Supplementary
Table S3). After aligning against the NCBI database, these four genes
were identified as <i>SaD17</i> (87% Ident), <i>SaD10</i> (83% Ident),<i>SaD14</i> (91% Ident) and <i>SaD53</i> (73% Ident) (Supplementary
Table S4). We further designed the qRT-PCR primers and optimized the
annealing temperature (Ta) for the qRT-PCR analysis (Supplementary Table
S4).</div>