Figure 5. The expression level of genes related to strigolactone biosynthesis and signal transduction in various tissues ofSpartina alterniflora . (a) SaD10 ; (b) SaD17 ; (c)SaD14 ; (d) SaD53 . The gene expression levels were analyzed by using the 2-△△CT method with SaTublin as reference gene. S: stem; N: basal node of the main stem; R: root; RH: rhizome; B: bud; L: leaf.
The expressional patterns of these four genes among different tissues including stem, basal node of the main stem, root, rhizome, bud and leaf in S. alterniflora were examined by using qRT-PCR. The quantitative results showed that SaD10 was mainly expressed in roots, SaD17 was mainly expressed in node and roots, SaD14was mainly expressed in leaf, root and node, while SaD53 was mainly expressed in leaf and node (Figure 5). Based on these results, we used roots to evaluate the expressional levels of SaD10 andSaD17 , and used nodes to evaluate the expressional levels ofSaD14 and SaD53 in response to salinities.