2.4 Experimental infection of mice
170
six-week-old
healthy BALB/c female mice were randomly divided into seventeen groups
of 10. Mice in PRV-injected groups were inoculated subcutaneously (s.c.)
with 500 μL different isolates respectively. Mice in DMED-injected group
were inoculated with 500 μL DMEM as control. After the inoculation, the
mice were examined daily for clinical signs for 7 days.