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Fig.1 Samples collection from Henan province of China for PRV detection during 2012 to 2019.
Fig.2 Positivity rate of PRV detection in different seasons (A) and regions (B).
Fig. 3 The isolation and identification of the PRV. A.The normal ST cell control;B. The ST cells infected with NY strain; C. Amplified PCR product of 429 bp; D. Electronograph of PRV NY strain
Fig. 4 Phylogenetic tree constructed by alignment deduced nucleotide sequences of gE (A) and gC (B) genes of the PRV isolates and reference strains using the neighbor-joining method. Bootstrapping with 1,000 replicates was performed to determine the percentage reliability for each internal node. Black triangles indicate PRV field isolates in this study.
Note:express the PRV strains in this study; ○express the Chinese re-emerging PRV strains (after 2012); express the early Chinese PRV strains (before 2012).